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petm11 expression vector addgene plasmid  (Addgene inc)


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    Structured Review

    Addgene inc petm11 expression vector addgene plasmid
    Petm11 Expression Vector Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/petm11+vector/pETM30-ARC105+(Plasmid+%23133426)/pm40301665-216-20-23
    Average 88 stars, based on 2 article reviews
    petm11 expression vector addgene plasmid - by Bioz Stars, 2026-09
    88/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Pervanadate-induced oxidation relieves autoinhibition of SRC protein tyrosine kinase
    Article Snippet: .. The cDNA encoding the human SRC SH2 domain (Gln147-Lys252) was subcloned into the pETM11 vector (a gift from Elisa Izaurralde (Addgene #145950)). .. The QuikChange II mutagenesis kit (Agilent) was used to construct the mutant SRC SH2 domains.

    Article Title: Engineering and Modulating Functional Cyanobacterial CO 2 -Fixing Organelles
    Article Snippet: .. The operons were inserted into either the pETM11 vector digested at the EcoRI and XhoI sites or the pAM2991 vector [a gift from Susan Golden, Addgene plasmid # 40248 ( )] at the EcoRI and BamHI sites. ..

    Article Title: Chemoenzymatic Generation of Lipid Membranes
    Article Snippet: This pathway, uses acetate as the starting precursor along with coenzyme A and is catalyzed by the enzyme acetyl-CoA synthetase (Acs; acetate:CoA ligase [AMP forming]), proceeding through an enzyme-bound acetyladenylate (acetyl-AMP) intermediate.100 Cells use this high-affinity pathway to scavenge for small concentrations of acetate.97,101 N-terminal His6-tagged ACS was expressed in E. coli and purified by adapting a previously published procedure.102 The pETM11 vector containing the ACS gene was purchased from Addgene that was deposited by the Schultz lab.102 To test the activity of ACS, we utilized two different assays. .. This pathway, uses acetate as the starting precursor along with coenzyme A and is catalyzed by the enzyme acetyl-CoA synthetase (Acs; acetate:CoA ligase [AMP forming]), proceeding through an enzyme-bound acetyladenylate (acetyl-AMP) intermediate.100 Cells use this high-affinity pathway to scavenge for small concentrations of acetate.97,101 N-terminal His6-tagged ACS was expressed in E. coli and purified by adapting a previously published procedure.102 The pETM11 vector containing the ACS gene was purchased from Addgene that was deposited by the Schultz lab.102 To test the activity of ACS, we utilized two different assays. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Pervanadate-induced oxidation relieves autoinhibition of SRC protein tyrosine kinase
    Article Snippet: .. The cDNA encoding the human SRC SH2 domain (Gln147-Lys252) was subcloned into the pETM11 vector (a gift from Elisa Izaurralde (Addgene #145950)). .. The QuikChange II mutagenesis kit (Agilent) was used to construct the mutant SRC SH2 domains.

    Purification:

    Article Title: Chemoenzymatic Generation of Lipid Membranes
    Article Snippet: This pathway, uses acetate as the starting precursor along with coenzyme A and is catalyzed by the enzyme acetyl-CoA synthetase (Acs; acetate:CoA ligase [AMP forming]), proceeding through an enzyme-bound acetyladenylate (acetyl-AMP) intermediate.100 Cells use this high-affinity pathway to scavenge for small concentrations of acetate.97,101 N-terminal His6-tagged ACS was expressed in E. coli and purified by adapting a previously published procedure.102 The pETM11 vector containing the ACS gene was purchased from Addgene that was deposited by the Schultz lab.102 To test the activity of ACS, we utilized two different assays. .. This pathway, uses acetate as the starting precursor along with coenzyme A and is catalyzed by the enzyme acetyl-CoA synthetase (Acs; acetate:CoA ligase [AMP forming]), proceeding through an enzyme-bound acetyladenylate (acetyl-AMP) intermediate.100 Cells use this high-affinity pathway to scavenge for small concentrations of acetate.97,101 N-terminal His6-tagged ACS was expressed in E. coli and purified by adapting a previously published procedure.102 The pETM11 vector containing the ACS gene was purchased from Addgene that was deposited by the Schultz lab.102 To test the activity of ACS, we utilized two different assays. ..

    Activity Assay:

    Article Title: Chemoenzymatic Generation of Lipid Membranes
    Article Snippet: This pathway, uses acetate as the starting precursor along with coenzyme A and is catalyzed by the enzyme acetyl-CoA synthetase (Acs; acetate:CoA ligase [AMP forming]), proceeding through an enzyme-bound acetyladenylate (acetyl-AMP) intermediate.100 Cells use this high-affinity pathway to scavenge for small concentrations of acetate.97,101 N-terminal His6-tagged ACS was expressed in E. coli and purified by adapting a previously published procedure.102 The pETM11 vector containing the ACS gene was purchased from Addgene that was deposited by the Schultz lab.102 To test the activity of ACS, we utilized two different assays. .. This pathway, uses acetate as the starting precursor along with coenzyme A and is catalyzed by the enzyme acetyl-CoA synthetase (Acs; acetate:CoA ligase [AMP forming]), proceeding through an enzyme-bound acetyladenylate (acetyl-AMP) intermediate.100 Cells use this high-affinity pathway to scavenge for small concentrations of acetate.97,101 N-terminal His6-tagged ACS was expressed in E. coli and purified by adapting a previously published procedure.102 The pETM11 vector containing the ACS gene was purchased from Addgene that was deposited by the Schultz lab.102 To test the activity of ACS, we utilized two different assays. ..



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